13 (D points) Bastase was immersed in a bulfer with p/t of 9 its catalytic react

Question

13 (D points) Bastase was immersed in a bulfer with p/t of 9 its catalytic reaction would stop at the A) (i) first tetrahedral intermediate, ( ii) H cannot act as an acid. 8) () second tetrahedral intermediate: () S cannot act as a nucleophile C) (0) D) (0) acyl-enzyme intermediate (i) D cannot act as a base econd tetrahedral intermediate: (i) H cannot act as an acid (0) second tetrahedral intermediate: () D cannot act as an acid 14)(8 points) On the tysoxyme reaction mechanism, the positively charge oxionium ion is stabilized by the presence nearby of A) E35 B) D 52 C) K 30 D) H 57 E) s 195 10/13/2015

Explanation / Answer

13. A. First tetrahydral intermediate because H cannot act as an acid. And the activity is lost at pH 9 like in case of high pH these enzyme has tendency to degrade or autolytic effect.

14. E 35 or Glutamine 35 stabilises the oxonium ions.It provides the stability to positive charge by forming the oxocarbonium ion.

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