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Question

Thank you! AaBbCcDdEe BCODE AaBbCDaE AaBbcDc AaBbcDdt AaBbC AasbsceE Heading 2 Tte Subtitle Normal No SpacingHeading 1 Draw the Michaelis-Menten plot and the Line Weaver Burk Plot using the data given below and find the Vmax and Km from the Plot. 3. Amount of Velocity Vol in maltose umoles/mn/mNo11 [5]in% [s] in milligrams in umoles 0.2 0.1 0.13 7.6 3.5 2.08 1.56 1.36 0.06 0.13 0.5 234 504 10 20 30 40 50 0.03 0.025 0.02 1152 1314 0.64 0.33 035 16719 00 0.77 4. Calculate the Activity of an amylase enzyme which is diluted 1:100 times with phosphate buffer and incubated for 1O minutes at 37 degrees Celsius. Given are the amount of maltose Img) 5.05 and the volume of enzyme used for the assay as 0.5m.

Explanation / Answer

3. since it is only 1 question per post i will only say that for question 3 you should start plotting the last 2 columns 1/S vs 1/V0 with that information you should be able to get the straightline and then you could get the values for km and Vmax

4.

enzyme activity = ugrams of enzyme x total dilution factor / (mw of substrate x incubation time x volume of enzyme)

ugrams of enzyme = 5.05 / 1000 = 5.05 x 10-3 ugrams

total dilution factor = 100

molar mass of maltose = 342 g/gmol

incubation time = 10 min = 600 segs

volume of enzyme = 0.5 ml = 0.0005 Liters

apply the equation

Enzyme activity =( 5.05 x 10-3 x 100 ) / (10 *0.0005*342) = 0.295 uM / min

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