3. A chromatogram was prepared using a procedare similar to Part I of this lab.

Question

3. A chromatogram was prepared using a procedare similar to Part I of this lab. solvent front origin line a) Measure the distance traveled by the solvent (cm preferred, but any unit will do). b) Measure distance traveled by substances A-E (in the same unit as the solvent front). c) Determine the retention factors for substances A-E B. Show you work for calculating the retention factor of A d) Calculate the retention factors for the two spots in each mixture (A/B and C/D) e) If you had a sample that was believed to contain two or three of the five substances measured above, could the solvent used to prepare the above chromatogram be used to identify the components of your unknown mixture? Why or why not?

Explanation / Answer

Distance travelled by the solvent front is the distance between the 'origin line' at the bottom and the 'solvent front' line at the top.

In order to measure the distance measured by a substance, you have to measure the distance of that particular substance's dot from the 'origin line'.

Retention factor for a substance = Distance travelled by the substance/Distance travelled by the solvent front

(e)

Since this solvent gives different retention factor values for all the five substances, hence it can be used to identify the components of the unknown mixtures.

Hope this helps !

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