Referring To The Beer-Lamb + https/ 30385 No results> Options Status Path: p Wor

Question

Referring To The Beer-Lamb + https/ 30385 No results> Options Status Path: p Words:0 QUESTION 8 Refer to the sections 18-2, 18-3, and 18-4 in the Harris textbook, specifically the Beer-Lambert-Bouguer law (the linear relationship between observed absorbance of monochromatic light and concentration of light-absorbing species). What might be the possible reason for absorbance readings (A) at a given wavelength (monochromatic light) having a nonlinear dependency on concentration of light-absorbing species? O The absorbances are so high 2) that the very few photons reach the detector. The detector has a finite sensitivity and cannot distinguish between these very small numbers of photons, thus 10 photons reaching the detector may give the same response as 5 photons. The sample has particles of insoluble matter / dust. The resulting light scattering (a.ka. Tyndall effect) biases the reading by increasing the observed absorbances (with only a part of the observed absorbance being due to true absorbance, and the rest - due to light scattering). O Molecules of the light-absorbing species participate in a concentration-dependent equilibrium (e.g., undergo a reversible association reaction, with the associated form having optical properties different from those of the free form). O Any of the above. QUESTION 9

Explanation / Answer

Option1: Absorbance >2 will lead to the nonlinear relationship between absorbance vs concentration. Hence, this option is correct.

Option2: If the sample contains large particle, due to the light scattering effect the number of photons detected by the detector is not uniform, which lead to a nonlinear dependency of a graph absorbance VS concentration. Hence, this option is also correct.

Option3: If the molecule has associated properties like aggregated form have different absorption than the monomeric state then there is a nonlinear dependency of absorbance VS concentration. This option is also correct.

Option 4: Any of the above, ( to have a nonlinear dependency of absorbance VS concentration any of the above options is correct.)

Hence, the correct answer is Option 4.

Hence, the correct answer is: Option 4 (Any of the above)

Related Questions

Navigate

• Browse (All)
• Browse R
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.