1- Which of the following is an enzyme often used prior to protein sequencing a.

Q: 1- Which of the following is an enzyme often used prior to protein sequencing a.

Answer:-1 Cyanogen Bromide is an inorganic compound used as a reagent in molecular biology. As a reagent for peptide mapping and amino acid sequence analysis, cyanogen bromide hydrolyzes peptide bonds at methionine residues in peptides and proteins. In addition, this agent acts as a coupling reagent for phosphoramidate or pyrophosphate inter-nucleotide bonds in DNA duplexes. so Cyanogen bromide is often used prior for protien sequencing.

ID: 578988 • Letter: 1

Question

Which of the following is an enzyme often used prior to protein sequencing

a. Cyanogen bromide

b. Trypsin

c. Sucrase

d. Mercaptoethanol

A dietary deficiency of what substance leads to the inability to synthesize

hydroxyproline and hydroxylysine

a. L-amino acids

b. D-amino acids

c. Collagen

d. Ascorbic acid

Phenylisothiocyanate is used to

a. reduce disulfides in proteins prior to electrophoresis

b. elute proteins from ion exchange column

c. combat the effect of nerve gas

d. derivatize the N-terminal amino acids of peptides for sequencing

Which of the following amino acids contains two nitrogen atoms?

a. Pro

b. Lys

c. Tyr

d. Arg

Hemoglobin is

a. A tetramer of 4 myoglobin proteins

b. A tetramer of 8 globin chains and one heme prosthetic group

c. A dimer of subunits each with 2 distinct protein chains (alpha and beta)

d. A dimer of subunits each with 2 myoglobin proteins

e. An erythrocyte

A biochemist works with a protein Y that is composed of several polypeptide chains.

He studies and ascertains the manner in which the several chain are bound together. He

has thus discovered the protein’s

a. primary structure

b. secondary structure

c. tertiary structure

d. Quaternary structure

7- A catalyst

a. increases activation energy

b. deacreases activation energy

c. promotes exothermicity

d. decreases free energy

After an enzyme has catalyzed a reaction several time, the quantity of the enzyme

within that cell will have

a. decreased

b. increased

c. remained unchanchanged

d. decreased, increased, remained unchanchanged depending on the reaction

Explanation / Answer

Answer:-1 Cyanogen Bromide is an inorganic compound used as a reagent in molecular biology. As a reagent for peptide mapping and amino acid sequence analysis, cyanogen bromide hydrolyzes peptide bonds at methionine residues in peptides and proteins. In addition, this agent acts as a coupling reagent for phosphoramidate or pyrophosphate inter-nucleotide bonds in DNA duplexes. so Cyanogen bromide is often used prior for protien sequencing.

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1- Which of the following is an enzyme often used prior to protein sequencing a.

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1- Which of the following is an enzyme often used prior to protein sequencing a.

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