Chemistry and Life in the Laboratory mo What do you conclude concerning the rela

Question

Chemistry and Life in the Laboratory mo What do you conclude concerning the relative abilities cules to pass through (permeate) the dialysis tubing? of protein and amino acid Account for this difference between proteins and amino acids 4. The Biuret Test Record the color for each solution and indicate whether it confirms a tive test. positive or a nega- Gelatin: Glutamic acid Egg albumin Bouillon cube: What chemical structure is required for a positive biuret test? Draw this structu re. 5. The Ninhydrin Test Indicate whether the solutions gave a positive or a negative test and record the color. Egg albumin: Bouillon: Alanine: What functional group is required for a positive test? Do both proteins and amino acids have this group? Explain.

Explanation / Answer

4. The Biuret Test:

Gelatin: Bluish violet colour will form

Egg Albumin: Bluish violet colour will form

Bluish violet colouration indicates the presence of protein!!

Glutamic Acid: Negative Test

5. The Ninhydrine Test:

Egg Albumin:  Intense blue colour will form

Alanine: Purple colored product

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