A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel el

Question

A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis. Assume there is no supercoiling. Answer the following three questions about the plasmid.

Can you please help below? I also don't understand it so a explanation for each would be helpful. For the first one I think its 1000bp but unsure. For the second, I think there is 2 HindIII sites and for the last one there is 1 EcoRI site? Please explain.

A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis. Assume there is no supercoiling. Answer the following three questions about the plasmid 2 3 4 Lane 1: DNA Ladder (100 bp increments beginning at 100 bp) Lane 2: EcoRI Lane 3: BamHI Lane 4: Hindll 2. What is the size of the plasmid? A. 100 bp B. 200 bp C. 500 bp D. 1000 bp E. 1500 brP How many Hindill sites are there in the plasmid? A. 0 3. C. 2 D. 3 E. Cannot be determined How many EcoRI sites are there in the plasmid? A. 4. C. 2 E. Cannot be determined

Explanation / Answer

2. The highest fragment is of size 1000bp. When cut by EcoR1, fragment of size 1000bp is found. No other fragment is there. Atleast two fragments should be in the gel, if atleast one site for EcoR1 is there. This means, that the plasmid has not been cut by EcoR1, and is in its supercoiled state in lane1. No site for EcoR1 is there.

The fragment is 1000bp

3. 2 HindIIIsites are there. (3 fragments mean two sites at unequal locations.)

4. 0 sites .

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.