A plasmid contains the gene for tetracycline resistance and a lacZ gene that has

Question

A plasmid contains the gene for tetracycline resistance and a lacZ gene that has a restriction site in it. Foreign DNA has the same restriction site at its ends. Host bacteria are transformed with the recombinant DNA and grown on medium with tetracycline and X-Gal. Colonies that are producing beta-galactosidase and are tetracycline resistant are carrying A) nothing. B) only lacZ. C) foreign DNA only. D) the intact plasmid only. E) the plasmid with foreign DNA.
A plasmid contains the gene for tetracycline resistance and a lacZ gene that has a restriction site in it. Foreign DNA has the same restriction site at its ends. Host bacteria are transformed with the recombinant DNA and grown on medium with tetracycline and X-Gal. Colonies that are producing beta-galactosidase and are tetracycline resistant are carrying A) nothing. B) only lacZ. C) foreign DNA only. D) the intact plasmid only. E) the plasmid with foreign DNA.

Explanation / Answer

Correct option is (D).

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