A Lineweaver Burk plot of the enzyme with increasing amounts of substrate in the

Question

A Lineweaver Burk plot of the enzyme with increasing amounts of substrate in the absence and presence of the inhibitor is shown below (Without inhibitor: x-intercept = -0.4, y-intercept = 0.2; With inhibitor: x-intercept = -0.4, y-intercept = 0.35). Calculate Km for the enzyme.

For the above question, calculate Vmax for the enzyme. Provide the numerical value and do not worry about units.

For the above question, what type of inhibition does the inhibitor work by? Provide your answer as competitive, uncompetitive, or noncompetitive.

Explanation / Answer

The inhibitor follow competitive inhibition

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