mRNAs have different half-lives in the cells. This is regulated, in part by sequ
ID: 59573 • Letter: M
Question
mRNAs have different half-lives in the cells. This is regulated, in part by sequences in the 3’ UTR, the elements are labeled as TGE. You place the mRNA shown in wild type cells, a cell containing a deletion of GLD1 and another with a version of GLD1 where the last 100 amino acids on the c-terminus of the proteins is removed. You then analyzed the polyA length and abundance of the mRNA.
a. What is the function of GLD1 in the cells?
b. What factors do you think GLD1 would bind to and bring to the mRNA?
c. Would deleting GLD1 increase translation of tra2? Defend your answer
d. Finally, what can you conclude about the C-terminus of GLD1
e. tough question: Suppose GLD1 is an essential gene and cells could not live without it. Is there a way to test if it regulates the mRNA even if you have to do experiments in cells containing the wild type gene?
Explanation / Answer
a)
GLD1 is a RNA binding protein, which specifically binds at 3'-UTR (untranslated) region with a consensus sequence 5'-UACUCAU-3' in an mRNA. This protein acts as a tumor suppressor protein, which is vital for oogenesis. The GLD1 controls the spatial patterns, expression and translation of mRNAs that are requited for oogenesis. Mutations in GLD 1 gene eliminate the function of tumor suppressor.
b)
The N-terminal domain of GLD1 recognizes the TGE sites and brings them to bind on mRNA for regulation of “Tra2 gene expression.”
c)
Usually the Tra2 gene expression is regulated (inhibition) of GLD1. So, deleting GLD1 gene results the increase expression or translation of tra2.
d)
The C-terminus of GLD 1 regulates the length of poly(A)tail. So, in the presence of GLD1 the poly A tail is short, vice-versa.
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