A 10 M solution of compound \"B\" is measured in a 1.00 cm path length cuvette a

Question

A 10 M solution of compound "B" is measured in a 1.00 cm path length cuvette at a wavelength of 500 nm. The percentage of the light transmitted through the cell is 38%. a)What is the absorbance of this sample (A) at 500 nm? b)Assuming Beer's Law holds, what is the value of E for compound B at 500 nm? c)A sample of compound B having an unknown concentration is measured to have an absorbance of .90 in a 1.00 cm path length cell at 500 nm. What is the concentration of B in this sample, assuming Beer's law holds?

Explanation / Answer

a) the absorbance A=log (1/T) where T =I/I(0) here t=38%=0.38 so A= log 1/0.38=0.42 b) A= epsilon*c*l usually you use l=1cm so l=1 0.42=0.1*epsilon and epsilon= 0.42/0.1=4.2/mole/cm c) 0.9=4.2*c so c=0.9/4.2=0.214M

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