Please show all your work and the thought process i.e why you did what you did,

Question

Please show all your work and the thought process i.e why you did what you did, also I have to do this by hand with a calculator, so please DON'T use graphs or plot anything. Thank you.

The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics:

V0 (mol/min)

—————————————

217

325

433

488

647

—————————————

Substrate added (mmol/L):

0.8

2

4

6

1,000

The Km for this enzyme is approximately:

A) 1 mM

B) 2 mM

C) 4 mM

D) 6 mM

E) 1,000 mM

Thank you for all your help. The correct answer is 2mM

Explanation / Answer

V0 = 217

S = 0.8

V = Vmax (S)/Km*(S)

V0 =Vmax/2

Vmax = V0 * 2

         = 217 *2

         = 434

Km = Vmax *(S)/(S) *V0

        = 434*0.8/0.8*217

      = 347.2/173.6 = 2mM

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