Given the following enzyme kinetic data, determine the K m , V max , and mode or
ID: 60135 • Letter: G
Question
Given the following enzyme kinetic data, determine the Km, Vmax, and mode or type of
behavior for ricamadene when sinnomore is metabolized by sinnomore hydroxylase.
Include the proper units with your numerical answer. Attached the L/B plot with the analysis
of the data. (Make sure that correct units are used for the calculations.)
rate of product formation (micromol/L per min)
rate of product formation in the presence of 0.1 micro M ricamadene
(micromol/L per min)
Km : __________________________________
Vmax : __________________________________
Behavior of ricamadene: __________________________________
BONUS: (determine if appropriate)
KI : __________________________________
KII : __________________________________
Sinnomore(nM)rate of product formation (micromol/L per min)
rate of product formation in the presence of 0.1 micro M ricamadene
(micromol/L per min)
2 149 21 4 233 37 8 338 55 16 482 79Explanation / Answer
Vo= Vmax *S/ Km+ S
482/ 338= 16( Km+8)/ 8(Km+16)
Km= 11.75nMPutting value of Km in the above equation, we can calculate Vmax
482= Vmax *16/ 11.75+16
Vmax= 835.96 micromol per lit per min
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