1.What process can be used to make a DNA probe produce the fluorescence of a che
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Question
1.What process can be used to make a DNA probe produce the fluorescence of a chemiluminescent signal?
A.
Splicing the gene for beta-lactosidase into the probe
B.
Substitution of biotinylated or fluorescent nucleotides into the probe
C.
Enzymatic attachment of acridinium esters to terminal ends of the probe
D.
Heat denaturation of the probe followed by acid treatment
2.In real-time PCR methods, how can products of multiple loci be identified?
A.
By simultaneous addition of hybridization probes to the master mix
B.
By following the increase in absorbance at 260nm during the method
C.
By labeling the primers with specific fluors
D.
By analysis of adenosine tail signatures
3.Which of the following statements concerning nucleotide synthesis is true?
A.
RNA can only be extended in the 2’ direction
B.
DNA or RNA can only be extended in the 3’ direction
C.
DNA or RNA can only be extended in the 5’ direction
D.
mRNA can be extended in the 3( or 2’ direction
CaNn you please answer these questions for me and explain why you choosed that answer
A.
Splicing the gene for beta-lactosidase into the probe
B.
Substitution of biotinylated or fluorescent nucleotides into the probe
C.
Enzymatic attachment of acridinium esters to terminal ends of the probe
D.
Heat denaturation of the probe followed by acid treatment
Explanation / Answer
1 B Substitution of biotinylated or fluorescent nucleotides into the probe.
Nick translation is the process used to introduce labeled nucleotides into the DNA. A DNase enzyme makes nicks whcih are repaired by Pol 1 enzyme. The enzyme uses labeled nucleotides to repair the DNA.
2.
C.By labeling the primers with specific colors.
By giving each primer a specific color we can track many products at the same time.
3.
C.DNA or RNA can only be extended in the 5’ direction
Both transcription and replication occur in the 5'-3' direction only. The enzymes work in this direction only
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