You are studying several alleles of an E. coli helicase gene. One alles, called

Question

You are studying several alleles of an E. coli helicase gene. One alles, called rs^R, conifers resistance to RS2014, an anitbiotic that works by inhibiting helicase activity. Bacteria with the rs^s allele are sensitive to RS2014. Another allele, called ts-, produces a temperature sensitive mutation of the helicase. In bacteria with the ts- allele, helicase is inactivated at 42 degrees C, but functions normally at 37 degrees. Helicase functions normally at both temperatures in baceria with the ts+ allele.

You obtain the following data while testing four strains of E. coli at different temperatures and doses of RS2014. Each number represens the % of maximal DNA synthesis. Based on this data, assign the appropriate genotype to strains A-D in the spaces provided.

You are studying several alleles of an E. col helicase gone. One allele, called ns, confers resistance to RS2014, an antibiotic that works by inhibiting helicease activity. Bacteria with the rs allele are sensitive to RS2014. Another with the ts' allele, helicase is inactivated at 42 but functions normally at 37 . Helicase functions normal at both temperatures in bacteria with the ts' alele. th allele, called ts, produces a tamperature sensitive mutation of the helicase. In becteria ho You obtain the following dsta while testing four strains of E, col at diferent t and doses of on this data, assign th RS2014. Each appropriate genotype to strains number represents the percentage of madimal DNA synthesis. D in the spaces provided Percent of Msoimel DNA synthesis |42 10 50 |37 10 50 0 |37 |37 0 |42 |42 Tomperature 37 37 37 42 42 42 RS2014 dose Temperature(Y Strain Genotpe A1 100 42 4 41 21 5 100 100 1100 | 10 100 1100 100 | 100 | 100 | 43 |42 | 41 100 43 6 100 43 7

Explanation / Answer

Strain A - rss ts- (rs susceptible and temperature sensitive)

Strain B - rsR ts+ (rs resistant and temperature resistant)

Strain C - rsR ts- (rs resistant and temperature sensitive)

Strain D - rss ts+ (rs susceptible and temperature resistant)

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