Napin promoter should only be expressed in seeds of Arabidopsis and Brossica pla

Question

Napin promoter should only be expressed in seeds of Arabidopsis and Brossica plants. This vector was designed to test seed specificity of Napin promoter in other plants and tissues - e.g. tobacco leaves. 1. Which transformation method can be used to preliminary assess possible expression from N promoter in tobacco leaves? 2. What type of transformation event / transformant cells we need for such analysis? 3. Can this transformation also result in the development of fully grown transgenic plants?

Explanation / Answer

1. Agrobacterial transformation method successfully to assess the possible expression of napin promoter in tobacco leages.

2. plant tissue is needed for this type of transformation.Assuming the presence of transformed DNA(T-DNA) marker, the cells to be cultured in a medium to produce shoots, roots atlast a transgenic plant. Arabidopsis and Brassica already transformed using the Agrobacterium tumefaciens.

3. The transgenic plant reproduces and grows like any other healthy non-transgenic plant.

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