How do you calculate the rate of change of absorbance per minute over the first

Question

How do you calculate the rate of change of absorbance per minute over the first 3 minutes to get units, U/ml, total units and percent recovery ? For lab we did an assignment to denature protein there was 3 salting out steps to get LDH and each time we took a measurement at 0 min, 1 min and 2 min. Therefore, I have 4 absorbance reading for each time. How do I figure out unit, U/mL, Total Units and % recovery. For example on the 1st readings we got: 0.130; 0.425; 0.573; 0.705. Can you please show me with these readings how to do the above.

Explanation / Answer

link 2: http://www.google.com/url?sa=t&rct;=j&q;=ldh enzyme lab, calculations&source;=web&cd;=1&cad;=rja&ved;=0CC4QFjAA&url;=http://www.mtholyoke.edu/~ndong20v/In our experiment.doc&ei;=fNsyUcbEMMLorQevpoEI&usg;=AFQjCNHZzYpMwc9B2RKFZZDOCUKOKv6Pag&bvm;=bv.43148975,d.bmk

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.