Question1 : If a sample of water from a pond is to be analyzed for whatever chem

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• Question1 : If a sample of water from a pond is to be analyzed for whatever chemicals it may contain (including organic compounds and metal containing species), give a very brief, but specific description of the protocol that would be best used, including what instrument(s) should be involved (choosing only from NMR and/or any chromatographic systems), and any specific accessories that might be needed (e.g. transducers/detectors, sample introduction system, etc.) as well as how the sample should be prepared for analysis, any interferences expected and how to overcome them, etc.

• Question 2: In most instrumental or analytical methods, use of an Internal Standard is considered the least desirable approach for calibrating the method. However, it is a very important technique for Chromatographic methods, especially GC. Why is this true?

• Questoin 3: The terms SCOT, WCOT, & PLOT are frequently used to describe types of GC columns. Specifically how do these columns differ from

Explanation / Answer

we can analyse the water sample by using different technique like GC, Mass, NMR, ESR, other.

column have differnt length or separation depending on the polarity and other conditions like hydrophobicity etc. in the case of GC , calibration is done internally because the set up parameters to check the sample is totally related to the internal factors and structure.

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