1) A histogram of a typical, freely dividing cell culture is shown in (A). (B) G

Question

1) A histogram of a typical, freely dividing cell culture is shown in (A). (B) Growth factor depletion can be used to "synchronize cells such that they are all in the same stage of the cell cycle. Show what the resulting histogram would look like following growth factor depletion. Why would growth factor depletion synchronize cells? (C) Propose a method or manipulation that could be used to synchronize cells in prometaphase and draw the resulting histogram for these cells 200- (A) 20 160 6120 80 5 40 E 40 0 200 400600 Fluorescence per cell (a.u.)

Explanation / Answer

1)

(A) Based on the given graph, as the cell number decreases the Fluorescence per cell (a.u) also decreases, vice-versa.

(B)

Synchronization of cell would be required during cell culture experiments of animal cell production, because process that helps to control or manipulate the all cells in the culture at a time. Therefore, the researcher can make sure about the cell size, density and fluorescent emission or Light scatter by labeled cells. One can bring the cells at different cell cycle phases into a single cell cycle phage by employing two types of Synchronization methods, namely

For example, in Chemical Blockade, depletion of growth factors that are exclusively required for growth makes the fast growing cells to slow down its dividing process. Therefore, all cells will Synchronize and present at the same stage.

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