eurodmin used in experiment 0-05 Exact mass of Part 1: Calibration Curve 1. Comp
ID: 636760 • Letter: E
Question
eurodmin used in experiment 0-05 Exact mass of Part 1: Calibration Curve 1. Complete the following table. Record the ??ax of curcumin: (should be close to 50 mg- 0.050 g) 10-nm Volume Stock Volume of Total VolumeConcentration Solution (mL) Ethanol (mL) Final Absorbance at ? (mL) 4.0 0.0 21 2.0m 3 1.5 ml 1.0 M? 4.0x105 2.0x103 1.5x10 1-249 2.5 ml 0.816 3.0m? 3.5 mL 10:10 0. 542 5.0x10*0. 318 2. Draw the absorbance spectrum for one of your standard samples. Indicate Amas on the curve. 40 sam 300 800 Wavelength (nm)Explanation / Answer
Sample
Absorbance
Initial Vol.
Final Vol.
Concentration of solution(graph)
Concentration of extract
1.
1.884
1 ml
1 ml
more than 4.00e-5
7.00e-5
2.
1.813
1 ml
2 ml
more than 4.00E-5
2.00e-5
Since, your absorbance cannot be found on the calibration curve, it should be more than 4.00e-5 or the same.(This data should be determined by extending the calibration graph) Let's assume that the concentration at 1.884 absorbance is 7.00e-5 M. (for making further calculations)
For the extracted solution, we can calculate the concentration of curcumin after dilution by M1V1=M2V2;
for the second sample, where dilution is made , (7.00e-5)*1 ml = M2 * 2 ml
hence, M2 = 3.50e-5.
Average concentration of original extracted solution would be, 3.50 * 10-5 M.
Since we know , that
C= (m/V )(1 / MW)
m= C* V * MW
m = 3.50e-5 * 5e3 * 368.4
milligrams of curcumin extracted =0.064 mg of curcumin
Mass percentage of curcumin = (0.064 mg / 59 mg ) * 100 = 0.001 % ( This could be different depending upon the concentration of curcumin from the calibration graph, which is not specified beyond 1.60 )
I hope the method helps! However, please check the values of the calibration graph to ensure the correct amount of extracted curcumin. If this helped, please rate it.
Sample
Absorbance
Initial Vol.
Final Vol.
Concentration of solution(graph)
Concentration of extract
1.
1.884
1 ml
1 ml
more than 4.00e-5
7.00e-5
2.
1.813
1 ml
2 ml
more than 4.00E-5
2.00e-5
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