Please answer ALL PARTS of questions 7-11. Thank you! Translation. 7. Let\'s say
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Please answer ALL PARTS of questions 7-11. Thank you!
Translation. 7. Let's say you infect E. coil with a double mutant phage then spread the E. cloi on an agar plate. The phage contains the RII mutation (KO) and a revert ant of FCC. Describe the plaque morphology (or morphologies) you expect to see on the agar dish? If you expect to see more than one morphology, explain which morphologies are expected to be rare and which are expected to be common. Explain your reasoning. 8. Now let's say you co-infect E. coil with the double mutant strain described above and WT phage (1:1 ratio). Now describe the plaque morphology (or morphologies) you expect to see on the agar dish? If you expect to see more than one morphology, explain which morphologies are expected to be rare and which are expected to be common. Explain your reasoning. 9. Consider the anticodon 5' 10. Based on what you know about anticodon/codon interactions and the wobble rules. What is the minimum number of tRNAs that can theoretically code for LEUCINE. 11. Write out the anticodon sequence (from 5' to 3') for each of these hypothetical tRNAs that you imagine are necessary to bind all leucine codons.Explanation / Answer
7. The phage contains a double mutant, so, the progeny phase would also be the double mutant. Since the double mutant is the result of the FC0 mutation and its revertant, then the phenotype produced would not contain any mutation and hence would be normal (wild-type). So, the morphology of the plaques looks like the wild-type plaques, depending on the bacterial strain on which they were grown.
8. The E.coli contains both double mutant phage and Wild type phage, so the resulting plaques show wild type appearance.
10. 6 tRNAs can code for LEUCINE.
11. UAA, CAA, AAG, GAG, UAG, and CAG
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