The enzyme fumarase catalyzes the conversion of fumarate to malate according to

Question

The enzyme fumarase catalyzes the conversion of fumarate to malate according to Use the data in the table above to determine the values of Rmax (the maximum rate of the enzyme, often called the maximum velocity, Vmax) and KM (the Michaelis-Menten constant of the enzyme). Answer the three parts below. a) If you were to plot this data to graphically determine Rmax and KM using a Lineweaver-Burk plot, what would you plot? If you would use the original data, enter the original value in the corresponding blank. c) Determine the values of Rmax (the maximum rate of the enzyme, often called the maximum velocity, Vmax) and KM (the Michaelis-Menten constant of the enzyme).

Explanation / Answer

Given below is the graph for calculation of Rmax and KM:

As per the graph, 1/Rmax = 0.078, Rmax = 12.9 = 13 ?mol/L.s

KM = 0.3026 x 13 = 3.9 ?mol / L

1/s (?mol. L-1) R (?mol. L-1. s-1) 1/S 1/R 1 2.6 1 0.38 2 4.3 0.5 0.23 5 7.2 0.2 0.14 10 9.3 0.1 0.11 20 10.8 0.05 0.09

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