In reverse genetics, what is the correct order in which the experimenter proceed

ID: 66298 • Letter: I

Question

In reverse genetics, what is the correct order in which the experimenter proceeds?

Selection of a phenotypic alternative for the gene in question and sequencing its DNA.

Silencing the genes in question using RNAi, followed by sequencing the gene.

Random bombardment of the DNA with a known mutagen, followed by observation of offspring with novel phenotypes

Screening genes via PCR, followed by deletion of the flanking sequences.

Creation of a mutant allele in a gene with unknown function, and then characterization of the mutant phenotype.

In reverse genetics, a specific gene or gene product is disrupted or modified and then the phenotype is measured.

So the answer is Random bombardment of the DNA with a known mutagen, followed by observation of offspring with novel phenotypes.

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