5) (2 points) The “suspect” DNA sequences used in this experiment are actually p

Question

5) (2 points) The “suspect” DNA sequences used in this experiment are actually plasmids (small, circular pieces of DNA that replicate in bacteria). The “restriction map” of one of the plasmids used as “suspect” DNA is shown below. A restriction map refers to the positions where restriction enzymes will digest a DNA molecule. Several different restriction enzymes are listed for this plasmid. The number after each restriction enzyme indicates the location of the restriction site relative to the “origin” of numbering of the plasmid (this plasmid is numbered 1 to 5869).

       a) How many DNA fragments arise when this plasmid is cut with both EcoRI and PstI? What are the sizes of these fragments?

Explanation / Answer

1)

Based on the given data, the sites of ECoRI and PstI are as follows:

Therefore, if we cut the plasmid with only ECoRI, then it will give one liner fragment with the size of 5869bp. If we cut the plasmid with ECoRI and PstI, then it will give five fragments. The Restriction map is as follows:

Thus, the sizes of fragments are:

2)

The gel of DNA sizes predicted in (a) is as follows:

The question does not give information of suspect gel. So, compare it with the above drawn gel.

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