Why is a protein’s conformation important for carrying out its function? What pr

Question

Why is a protein’s conformation important for carrying out its function?

What properties of the amino acids in a protein relate to protein folding?

Does the eluate containing your red fluorescent protein appear less bright
or brighter than it did in the cell lysate following centrifugation? If there is a noticeable difference in the intensity of the red color, what might account for that?

What characteristic of red fluorescent protein is used as the basis for separation by column chromatography?

How might the column chromatography procedure be adjusted or modified to increase the purity of the red fluorescent protein sample?

Explanation / Answer

A protein's conformation tells you what its promoter is.

Amino acids tell how the protein will fold.

The RFP eluate is bighter than it was in the cell lysate because we diffused the unwanted protein out.

When unfolded the RFP stick to the resin column.

To increase the purity of the RFP, we could continue to wash it in column chromotography.

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