Transcribe and translate this piece of DNA into a protein. Suppose there is a mu

Question

Transcribe and translate this piece of DNA into a protein. Suppose there is a mutation in the DNA. Suppose once base pair changes. What would be the result? Original DNA: Mutated mRNA: Mutated Polypeptide: Is the mRNA the same as question 20's mRNA? Is the protein the same as question 20's protein? What kind of mutation is this? (two answers acceptable!) Suppose there is a mutation during transcription. Suppose the AT In GAT is not transcribed. What is the new mRNA sequence? What is the new amino acid sequence? original DNA: Mutated mRNA: Mutated Polypeptide: Is the mRNA the same as question 20's mRNA? Is the protein the same as question 20's protein? What is different about the protein? What kind of mutation is this? (two answers acceptable')

Explanation / Answer

20. 3' TAC CAT GAT CAT CAA TAG CGA ATT = 5'

The mRNA 5' - AUG GUA CTA GUA GUU AUC GCU UAA 3'

polypeptide - Met V L V V I A Stop

21. 3' TAC CAT GAT CAT CAC TAG CGA ATT = 5'

mRNA 5' - AUG GUA CTA GUA GUG AUC GCU UAA 3'

mutant polypep - Met V L V V I A Stop

The mRNA is not the same of question 20 but the protein is the same it is called as silent mutation.

22. mRNA  5' - AUG GUA CGU AGU GAU CGC UUA A 3'

mutant poly pep Met V R S D R L

the sequence of both mRNA and polypeptide is changed. It is called as deletion mutation.

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