Hi there! I would like to check if my understanding of (b) and (c) is correct. (

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Hi there! I would like to check if my understanding of (b) and (c) is correct.

(b) For studying the kinetics of protein adsorption, I would say that I would use the Bradford assay.
The Bradford assay is uses Coomarie Brilliant Blue G250 to identify the proteins and the intensity of the colour change relates to the amount of protein adsorbed.

I would like to ask, are there any alternative methods beside Bradford assay to determine protein kinematics?

And for (c), protein adsorption relates to the hydrophobicity of the material.
To decrease protein adsorption, I will need to make the surface more hydrophilic.
Thus, I will use the Self-Assembled Monolayer technique, which has a polar carboxylic acid group exposed to the environment, to make the surface more hydrophilic.
(An alternative method would be to use radiation grafting to coat a layer of hydrogel to the substrate)

Einrofollowing information of the material: crystal structure, elements present (8 marks) You want to study the kinetics of protein adsorption on this material. n its surface, hydrophobicity and surface roughness. (b) tri , mam Which analysis technique would you use for this purpose? How does it work to obtain the kinetics of protein adsorption? natourd pelrea c ti e l w (6 marks) (c) Your experiment shows that blood proteins exhibit strong adsorption on this material. Suggest a surface modification method to minimize the protein adsorption and explain its working mechanism. (8 marks) 3. An investigator wishes to identify a sample containing different isoforms of a protein. The sample mixture which contains four isoforms of protein X were ran through a reverse-phase HPLC system utilizing methanol-water as the mobile phase. The following chromatograph shows the sequence of elution. Chromatogram

Explanation / Answer

b) your understanding of the question is preety fair nad the approach is also correct.
Although I would like to add that instead of bradford method silver staining of protein can also be used which is almost 10 times more sensitive than Bradford assay and can detect <1 ng of protein. Silver staining utilises the protein binding properties of silver ions, which are then reduced to silver metal using a developing solution, creating a visible image.

c) The dynamics of protein adsorption is a complex phoenomenon taking in account a variety of factors. But hydrophobicity of the surface is definetely one of the key players in the science of protein adsorption. So, I totaaly agree with your point of making the surface hydrophilic to reduce protein adsorption. Smart idea indeed.

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