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In the lac operon, what are the likely effects on operon gene transcription of t

ID: 67590 • Letter: I

Question

In the lac operon, what are the likely effects on operon gene transcription of the mutations identified below?

Mutation of consensus sequence in the lac promoter

Mutation of the repressor binding site on the operator sequence

Mutation of the lac I gene affecting the allosteric site of the protein

Mutation of the CAP binding site of the lac promoter

Mutation of the lac I gene affecting the DNA-binding site of the protein

A. Transcription is blocked.

B. Transcription is constitutive.

C. No effect on transcription

Explanation / Answer

Mutation of consensus sequence in the lac promoter -----------> A. Transcription is blocked.

Mutation of the repressor binding site on the operator sequence -----------> B. Transcription is constitutive.

Mutation of the lac I gene affecting the allosteric site of the protein -----------> A. Transcription is blocked.

Mutation of the CAP (catabolite activator protein) binding site of the lac promoter -------------> A. Transcription is blocked.

Mutation of the lac I gene affecting the DNA-binding site of the protein ------------> B. Transcription is constitutive.

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