For E. coli with the following genotypes, indicate whether lacZ and lacY (give a

Question

For E. coli with the following genotypes, indicate whether lacZ and lacY (give a separate answer for lacZ and lacY) are inducible, constitutively expressed, or non-inducible (i.e., can’t be expressed).

a)         lacI+ lacP+ lacO+ lacZ- lacY+ / lacI- lacP+ lacO+ lacZ+ lacY-

b)         lacI+ lacP+ lacOC lacZ- lacY+ / lacI- lacP+ lacO+ lacZ+ lacY-   

c) lacI+ lacP+ lacOC lacZ- lacY+ / lacIS lacP+ lacO+ lacZ+ lacY-

d)         lacI+ lacP- lacO+ lacZ- lacY+ / lacI- lacP+ lacO+ lacZ+ lacY-

Explanation / Answer

a) Here both Lac and lacY are inducible

b) Here lacZ is inducible and lacY is constitutively expressed

c) lacY is constitutively expressed and lacZ is non-inducilbe because of super-repressor mutation

d) lacY is non-inducible and lacZ is inducible

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