The specificity of the PCR reaction, making millions of copies of only a relativ
ID: 67978 • Letter: T
Question
The specificity of the PCR reaction, making millions of copies of only a relatively small region of DNA, is primarily due to a. A thermostable DNA polymerase. b. Synthetic DNA primers. c. 95°C melting temperature d. The 4 Dideoxy Nucleoside triphosphates. e. Template DNA. 2. Which of the following is NOT required for an efficient DNA cloning vector replicating in bacteria? a. Circular DNA. b. Origin of replication. c. Telomeric end sequences. d. Antibiotic-resistance gene. e. Many unique restriction enzyme cutting sites. 3. 4. A gene library Can be made from random fragments of genomic DNA, or only expressed sequences of DNA Is a collection of bacterial cells, each containing a different piece of an organism's genetic information. Is often produced using restriction enzymes, engineered vectors, and DNA ligase. Can be screened by specific sequences using PCR or colony hybridization. All of the above. a. b. c. d. e. A "word" in the genetic code is directly recognized and read by a. The ribosomal small subunit b. The ribosomal large subunit c. The anticodon on the tRNA d. The DNA template e. Excision repair "seeker" proteins 5.Explanation / Answer
2. The specificity is due to the synthetic primer. So, the answer is (b).
3. Telomeric end sequences are not required as the end part is not open because of being circular. So, the answer is (c).
4. Ans. (e). All of the above.
5. The genetic code is read during translation via adapter molecules, tRNAs. So, the answer is (c).
6. Electrophoresis do distinguish the DNA band of different molecular size but a band may contain two segments of the same size. Therefore, in order to confirm the gene of interest, in that case, the technique proceeds with transferring the fragment to a filter membrane and the subsequently gene of interest detection by probe hybridization, a process termed as Southern blotting. So, the answer is (b).
7. None of the options is right.
8. During replication DNA polymerase causes replication and RNA polymerases causes polymerization during transcription. So, the answer is
9. Transcription is initiated at promotor region. So, the answer is (d).
10. Translation is terminated by a protein release factor which recognises the termination codon. So, the answer is (a).
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