5.Answer the following dilution problems (include the volumes ofstock solution a

Question

5.Answer the following dilution problems (include the volumes ofstock solution and diluents)

a) Make 25 ml of 20% ethanol from a 95% ethanolstock.

b) Make 500 ml of 1X phosphate buffered saline (PBS) from a20X PBS stock.

c) Make 20 ml of 0.5% NaCl from a 10% stock.

d) How many mL of a 5.0% glucose solution provide 80.0 g ofglucose?

Explanation / Answer

a) c1*V1= c2*V2, hence 0.95*V1= 25*0.2 => V1= 5.26 ml stock and19.74 ml diluent b)500/20= 25 ml stock and 475 ml diluent c)0.1*V1=0.05*20 => V1= 10 ml stock and 10 ml diluent d) I assume it is 5 % (weight/volume) hence 5 g/ml => 80/5= 16ml 5 % glucose solution

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