The expression of proteins in E. coli under control by the lac repressor can be

Question

The expression of proteins in E. coli under control by the lac repressor can be induced with IPTG when the cells grow to a density of about O.D. 0.600 at 600 nm. When you express proteins in E. coli, you need to monitor the growth by measuring the absorbance of the culture and induce the express by adding IPTG. Recently a new auto-induction technique was developed using a media containing 0.05% glucose and 0.2% lactose. The expression of the protein will be induced automatically at the right cell density in overnight cultures. Please explain how this system works

Explanation / Answer

The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia coli without the need to monitor the culture or add inducer during cell growth.

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