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Glycolysis, ETC, and Krebs cycle biochemistry product questions: (This is bioche

ID: 68711 • Letter: G

Question

Glycolysis, ETC, and Krebs cycle biochemistry product questions:

(This is biochemistry)

1. If you created an artificial mitochondria and injected 1 molecule of pyruvate inside it, how many protons would accumulate in the intermembrane space of the mitochondria (starting from 1 molecule of pyruvate take it all the way to the end of the electron transport chain).

46

92

104

32

2. If you have created an artificial mitochondria and are injecting ONE molecule of Pyruvate inside it, how many molecules of ATP can be theoretically generated starting from 1 molecule of Pyruvate ( start from pyruvate and complete all the reactions to finish oxidative phosphorylation). Remember you also need to take into account any ATP generated via substrate Level phosphorylation.

11.5

12.5

32

30

3. If you have generated an artificial mitochondria and have injected 1 molecule of Acetyl CoA inside it, how many protons can theoretically accumulate in the intermembrane space of the mitochondria at the end of electron transport chain?

46

32

46

36

4. If you have generated an artificial mitochondria and have injected 1 molecule of Acetyl CoA inside it, how many ATP molecules can theoretically accumulate in the intermembrane space of the mitochondria at the end of electron transport chain and oxidative phosphorylation? ( Remember to take into accound ATP molecule generated via Substrate Level Phosphorylation)

12.5

9

10

32

5. You have an artificial mitochondria, and you inject into it 10 molecules of a compound X that can donate a pair of electrons to complex III of the mitochondria, how many protons can theoretically accumulate in the intermembrane space of the mitochondria ?

60

100

104

112

46

92

104

32

Explanation / Answer

1. Answer is 92. One molecule of pyruvate when injected in mitochondria, it produces 92 protons in the intermembrane space of the mitochondria. the pyruvate is imported into the mitochondrion and oxidized by O2 to CO2 and H2O

2. Answer is 11.5.One molecule of pyruvate can generate 11.5 ATPs ( 4 NADH x 2.5 ATP = 9 ATPs and 1 FADH2 x 1.5 = 1.5 ATPs)

3. Answer is 36. Acetyl CoA gives 3 NADH+ and FADH2, so altogether it will give 36 protons.

4. Answer is 12.5. 1 molecule of Acetyl CoA can generate 12.5 ATP

5. Answer is 60. When the molecule of compound X can donate a pair of electrons directly to complex III, for a pair of electrons it gives 6 protons, so 60 protons are releases from 10 molecules.

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