A solvent passes through a chromatography column in 3.5 minutes, but the solute
ID: 696320 • Letter: A
Question
A solvent passes through a chromatography column in 3.5 minutes, but the solute requires 9.8 minutes. (a) What is the retention factor, k? (b) What fraction of the time does the solute spend in the mobile phase in the column? Number 110.181 Number 11 1.74 fraction in mobile phase The volume of the stationary phase is 0.16 times the volume of the mobile phase in the column. (Vs 0.16Vm-) (c) What is the partition coefficient, K, for this system? Number K= | | 10.875 There is additional feedback available! View this feedback by clicking on the bottom divider bar. Click on the divider Incorrect bar again to hide the additional feedback. CloseExplanation / Answer
1)
Retention factor = total time - time taken by solvent /time taken by solvent
= 9.8 - 3.5 / 3.5
retention factor , k = 1.8
2)
Fraction of the time the solute spends in the mobile phase = time spent by solvent / total time
= 3.5 / 9.8
Fraction of the time = 0.36
3)
Partition coefficient = 1.8 / 0.16 = 11.25
partition coefficient = 11.25
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