Now you Study enzyme inhibition by measuring enzyme kinetics in the presence of

Question

Now you Study enzyme inhibition by measuring enzyme kinetics in the presence of 10 mM of inhibitor A or inhibitor B (separately). The Lineweaver-Burk plots in the presence of these inhibitors are indicated by "+A" or "B" in the Figure below. What Type of inhibitor of A and B is show in the figure above ( Competitive, pure noncompetitive, mixed non-competitive or uncompetitive) Inhibitor A: Inhibitor B: What effect (increase, decrease, stay the same) does the inhibitor depicted below have on Vmax Effect of inhibitor A on Vmax: Effect of inhibitor B on Vmax: What effect (increase, decrease, stay the same) does the inhibitor depicted below have of Vmax Effect of inhibitor A on Km: Effect of inhibitor B on Km:

Explanation / Answer

1)

Inhibitor A: competitive

Inhibitor B: mixed non-competitive

2)

Competitive inhibitor binds to the enzyme’s actives site. It affects the Km, but will not affect Vmax.

Non-competitive inhibitor lowers the Vmax.

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