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Question

You will be graded based on your ability to identify formulate, and solve engineering problems. Clarity of presentation would ensure that you do not lose all the points for a calculation if you should make a mistake 1. 1.0 ml of a preparation of the enzyme glucoamylase was added to 9 ml of a starch solution to give a final enzyme protein and starch concentrations of 0.8 mg protein/ml and 4 %wt, respectively and a pH 4.5 and temperature 60 oC If initial rate experiments show that the glucoamylase catalyses the production of 0.6 umol of glucose/ml-min, calculate the specific activity of the glucoamylase preparation 2. Lactase, also known as p-galactosidase, catalyses the hydrolysis of lactose to produce glucose and galactose from milk and whey. Initial rate data below were obtained from experiments carried out to determine the kinetic parameters for the enzyme actose concentration (mol I 10 Initial reaction velocity (mel I'minx 10 2.5 2.27 1.84 1.94 1.91 1.85 1.80 1.25 0.730 0.460 0.204 1.46 1.17 0,779 ) Using the initial rate data present (a) Lineweaver-Burk and (b) Hanes plots (b) From the two plots calculate: o the V, n the K (i)k, given that E-0.015 g aüi) Comment on the values of Vma, K, and k obtained using the Lineweaver-Burk and (b) Hanes plots

Explanation / Answer

The Lineweaver Burke plot is basically aan extension of the normal MM kinetics curve

It may be easily evaluated from a straight line plot.

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