Question VII. While working on an adenovirus vaccine, you perform complement fix
ID: 70284 • Letter: Q
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Question VII. While working on an adenovirus vaccine, you perform complement fixation assays to determine semiquantitatively the titers of anti-viral antibody in the serum of subjects following vaccination. You are using the typical version of CFT, in which you add a standard amount of viral antigen and complement to serial dilutions of the subjects’ sera, in which complement was heat-inactivated and then adding sRBC. Your results for three subjects are shown below:
Based on these results, assess each subsequent statement (56-62) as either factually correct (= “true”) or incorrect (= “false”)]:
56. Subject #1 has probably more anti-viral antibody than #2.
57. Subject #1 has probably less anti-viral antibody than #2.
58. Subject #1 has probably more anti-viral antibody than #3.
59. Subject #1 has probably less anti-viral antibody than #3.
60. Subject #2 has probably more anti-viral antibody than #3.
61. Subject #2 has less anti-viral antibody than #3.
62. None of the above 6 statements (56-61) is a valid conclusion.
Lysis with added antigen Lysis with Test Sample Dilution #1 no added antigern 1:10 1:20 1:40 1:80 12 1:10 1:20 1:40 1:80 #3 1:10 1:20 1:40 1:80Explanation / Answer
60. Subject #2 has probably more anti-viral antibody than #3.
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