Question VII. While working on an adenovirus vaccine, you perform complement fix

Question

Question VII. While working on an adenovirus vaccine, you perform complement fixation assays to determine semiquantitatively the titers of anti-viral antibody in the serum of subjects following vaccination. You are using the typical version of CFT, in which you add a standard amount of viral antigen and complement to serial dilutions of the subjects’ sera, in which complement was heat-inactivated and then adding sRBC. Your results for three subjects are shown below:

Based on these results, assess each subsequent statement (56-62) as either factually correct (= “true”) or incorrect (= “false”)]:
56. Subject #1 has probably more anti-viral antibody than #2.

57. Subject #1 has probably less anti-viral antibody than #2.

58. Subject #1 has probably more anti-viral antibody than #3.

59. Subject #1 has probably less anti-viral antibody than #3.

60. Subject #2 has probably more anti-viral antibody than #3.

61. Subject #2 has less anti-viral antibody than #3.

62. None of the above 6 statements (56-61) is a valid conclusion.

Lysis with added antigen Lysis with Test Sample Dilution #1 no added antigern 1:10 1:20 1:40 1:80 12 1:10 1:20 1:40 1:80 #3 1:10 1:20 1:40 1:80

Explanation / Answer

Answer question

56 is false

57 is true

58 is false

59 is true

60 is true

61 is false

62 is false

CFT TEST RESULTS ARENEGATI WHEN HEMOLYS IS SEEN BZ an and At is not fixed with compliment and in positive reaction An and Age is fixed so no hemolysis is seen

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