The antibiotic erythromycin disrupts protein synthesis by preventing ribosomal t

Question

The antibiotic erythromycin disrupts protein synthesis by preventing ribosomal translocation. It does not affect any other step. Suppose you are doing a translation reaction in vitro and you add erythromycin just before the 3rd bond is about to be made. You wait a few minutes and look at the ribosome. a. The carboxyl end of amino acid #3 should now be covalently bonded directly to (circle the correct answer and explain your answer below).

i. tRNA #2

ii. tRNA #3

iii. tRNA #4

iv. Amino acid #2

v. Amino acid #3

vi. Amino acid #4

vii. None of the these

Explanation / Answer

The carboxyl end of amino acid #3 should now be covalently bonded directly to Amino acid #4. Initially, the aminoacid #3 is attached to tRNA #3, but as the growing polypeptide got transferred to A site before translocation, it bonded directly to Amino acid #4. Peptide bond formation can still occur even in the presence of erythromycin.

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