Its urgent, I would really appreciate the help. I will rate you for corect answe

Question

Its urgent, I would really appreciate the help. I will rate you for corect answers.

QUESTION 1 What is the function of SYBR in real time PCR?

a. Activator b. Reporter c. Promotor d. Inhibitor

QUESTION 2 Which template is used to do real time PCR?

a. mRNA b. genome DNA c. cDNA d. not sure

QUESTION 3 Real time PCR can monitor the expression of certain genes in early stage.

True False

QUESTION 4 Using capillary electrophoresis-based Sanger sequencing, how long will the Human Genome Project took?

a. Over 3 years b. Over 5 years c. Over 10 years d. Over 15 years

Explanation / Answer

1. What is the function of SYBR in real time PCR?

SYBR is a dye which is used in nuclear acid stain in molecular biology experiments. Due to its binding to DNA, the DNA-dye complex absorbs blue light and emitts green light. Like ethidium bromide it is also mutagenic. It acts as a fluorescent reporter.

The correct option is ...................b.reporter

2. Which template is used to do real time PCR?

In mRNA based polymerase reactions, reverse transcriptase acts to convert RNA to cDNA. This cDNA is used as template in real time PCR to quantify the RNA.

The correct option is........................c. cDNA

3. Real time PCR can monitor the expression of certain genes in early stage.

Real time PCR is useful in monitoring the expression of genes through quantitative real time PCR. It needs to reverse transcribe the mRNA to cDNA and needs to amplify the cDNA to measure the gene expression.

The correct option is ..................................True

4. Using capillary electrophoresis-based Sanger sequencing, how long will the Human Genome Project took?

There were 3 billion base pairs (haploid), costs about 3 billion dollars to sequence the first genome, it took about 13 years (2000-2013) to complete the genome project.

The correct option is ..................c. Over 10 years

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