Some plase answer multiple choice qustions 87 to 90 The polymerase chain reactio

Question

Some plase answer multiple choice qustions 87 to 90

The polymerase chain reaction (PCR) requires: Taq polymerase, a primer set, the four deoxynucleotides, and a template Taq polymerase, a primer, template, the four dexoynuclotides and one dideoxynucleotide Taq polymerase, a primer set, four labeled deoxynucleotides, and a template DNA polymerase, a primer, ligase, and the four deoxynucleotides Taq polymerase, a primer set, ligase and the four deoxynucleotides The following diagram represents a PCR reaction after the annealing step. Which of the following correctly corresponds to numbers 1, 2, 3 and 4, respectively? forward primer, reverse primer, 3'OH, 5'P forward primer, reverse primer, 3' OH, 3'OH reverse primer, forward primer, 5' P, 5'P reverse primer, forward primer, 3'OH, 5'P forward primer, reverse primer, 5'P, 3'OH From the following agarose gel electrophoresis result, which of the DNA fragments numbered 1 to 5, is the smallest fragment? 1 2 3 4 5 Which of the following is not used for DNA sequencing? a primer DNA polymerase nucleotides restriction endonucleases fluorescent or radioactive labels The following strand of DNA is to be used for sequencing:

Explanation / Answer

ans 87-C

ans 88-A as primers synthesize new strand only in 5 prime to 3 prime direction

ans 89-E as DNA is negatively charged so it will move towards positive electrode

ans 90-D restriction endonuclease is not used

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