Not a super hard question but I really don\'t get it. Only answer if you can acc

Question

Not a super hard question but I really don't get it. Only answer if you can accurately answer all parts, especially D PLEASE :)

This question is for biochem 311...

Using the diagrams below, indicate the position of tRNAs

(A) during tRNA selection before GTP hydrolysis by EF-Tu,

(B) after GTP hydrolysis by EF-Tu and proofreading,

(C) after peptide bond formation but before translocation, and

(D) after translocation by EF-G. Indicate the tRNAs as straight lines and the nascent peptide by a squiggly line. Which one of these represents the hybrid state? Finally, indicate which steps involve GTP hydrolysis.

Explanation / Answer

(A) During tRNA selection before GTP hydrolysis by EF-Tu – at position P

(B) After GTP hydrolysis by EF-Tu and proofreading – at position P and A

(C) After peptide bond formation but before translocation, and – at position P and A

(D) After translocation by EF-G – at position E and P

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