I WOULD ONLY LIKE TO KNOW HOW TO FIND GLUCOSE CONCENTRATION IN PART C , thanks!
ID: 716924 • Letter: I
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I WOULD ONLY LIKE TO KNOW HOW TO FIND GLUCOSE CONCENTRATION IN PART C, thanks!
4. A cell culture is grown on a medium containing 20mM of glucose. At time- 0, it is inoculated into a larger sterile volume of the identical medium. During the course of the batch fermentation, the cell density was measured; the readings are given below Time Cell density/mL 0 1.00E+05 1.10E+05 1.20E+H05 1.54E+05 1.98E+05 2.54E+05 4.19E+05 6.91E+05 1.14E+06 1.88E+06 3.09E+06 3.50E+06 3.60E+06 15 20 25 35 45 65 75 85 95 A) Plot the ln of cell density vs. time, label each phase of growth, and estimate the lag time for this fermentation. B) Evaluate the specific growth rate, the doubling time, and the overall yield of cells (as number of cells per gram of substrate) assuming complete substrate utilization during the batch C) Plot the glucose concentration vs. time.Explanation / Answer
U can find concentration of glucose by given cell density.
Cell density = no of cells per unit volume
Hence different concentration of glucose can be find by different cell densities given.
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