You have made a 20 mM succinate buffer at pH 5.64 in a 2.0 mL solution to run an

Question

You have made a 20 mM succinate buffer at pH 5.64 in a 2.0 mL solution to run an enzyme reaction...

error in calculation or pH meter) ror the unexpected pri clage 6) (2 points) You have made a 20 mM Succinate buffer at ptH 5.64 in a 2.0 ml solution to run an enzyme reaction which generates H'. When you finish the reaction, you find the pH has shifted to a pH of 4.0. Much lower than you would like. How would you adjust your buffer to avoid such a significant pH change? You must use succinate buffer and because of the enzyme's optimal pH requirement you must maintain a pH of 5.64. No calculations are required for this answer, just identify the problem and describe how/what you would do differently There is a key buffer concept for this question you should identify in your answer

Explanation / Answer

Answer:
To adjust the buffer pH and to resist the change in a pH, a small amount of acid and base is added. They can do this because they contain both acidic and basic component, hence they can neutralize the opposite ion.

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