The gene (cv) is recessive and is known to be 13.7 Map units from the beginning

Question

The gene (cv) is recessive and is known to be 13.7 Map units from the beginning of the chromosome. The recessive gene (f) is 51.7 map units from the beginning of the chromosome. After doing all of the calculations i found my gene order to be we-cv-f. Gene (we) is the unknown gene which i mapped to be 17.2 map units from gene(cv). This puts the unknown gene off of the chromosome by 3.5 map units.

Why is this? What are some possible genetic explanations for this?

**Please assume all calculations are correct and when this experiment was performed around 400 fly's phenotypes were scored.

Explanation / Answer

I think it should actually be cv-we-f a possible genetic explanation for this is that there are certain hotspots on a chromosome that are more likely to break off and exchange (please excuse my vocabulary) with its twin during meiosis. Genes are mapped based on how often they are inherited together and we assume a linear relationship between distance between genes and the % of times they're inherited together and our measurement units are based on that. That is not always true and I think there might be such a hotspot near the end of the chromosome, where the unknown gene is likely to be.

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