If you are asking which method to use to check whether the gene has been knocked

Question

If you are asking which method to use to check whether the gene has been knocked out or not. This can be done by using gene expression. To know which method is used to generate a knockout mouse, it is RNAi also called RNA interference. I do understand this but i am asking Gene of unknown function in the mouse genome was investigated with reverse genetic approach Knock out mouse was created was missing particular gene When phenotype of investigated mouse was compared the only difference from the wild-type was mouse kicking other mouse And I know the protein encoded was named KST So my question is what are the some of aspects of the KST protien that could be investigated in order to better understanding the biology ?

Explanation / Answer

Kicking is a behavior, not a trait. While behavior is sometimes studied this way, it is usually only done in a particular area of research which has a specific genetic cause. For example, mouse models for Alzheimer's disease (which is a behavior) have been studied by interrupting certain neurotransmitters (genetic basis). Unless it was some kind of unusual or extreme kicking (which you should note in the question if that's the case), wild type mice are likely to kick just the same as your experimental mice.

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