Write a paper that includes the following: 1. Title page: descriptive title, you

Question

Write a paper that includes the following:

1. Title page: descriptive title, your name, course name, semester

2. Introduction: general background information about enzymes and specific information about your chosen enzyme, the question(s) that you are asking and a clear hypothesis for your experiment (20 points).

3. Design an experiment. Provide a detailed description of the materials and methods used to conduct the experiment. Identify control and experimental samples, as well as independent and dependent variables. Also include the methods used for data collection and analysis (20 points).

4. Conduct the experiment and record your results. What did you observe? Present your data in table and/or graph format. Remember to label everything and include the unit of measure with all numbers (20 points)

5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary for you to refer to the OLI course modules, lab manual and/or use additional information resources. What effect does the acidic treatment have on enzyme activity? Did you get the expected results? Explain. (20 points)

6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back, how could you have improved your experiment? (10 points)

7. Include a list of references to all information sources used in APA format (5 points).

Topic: The Effect of a Catalase on the Breakdown of Hydrogen Peroxide

Explanation / Answer

The effect of PH on catalse in breakdown of hydrogen peroxide

NAME:

COURSE NAME:

SEMESTER:

INTRODUCTION: Enzymes are macromolecular biological catalysts. Enzymes accelerate, or catalyze, chemical reactions and remains unchanged at the end of the reaction. The molecules at the beginning of the process are called substrates and the enzyme converts these into different molecules, called products. The activity and efficiency of enzymes depends on temperature and pH conditions. Catalase is one of antioxidant enzyme which breaksdown hydrogen peroxide (H2O2) into oxygen and water. Catalase is found in a cell organelle called the peroxisome. It is essential to know the pH condition at which catalse acts most effectively. If the pH the catalase is subject to is acidic (low pH) then there will be more enzyme activity than if it were subject to a base, because the enzyme catalase is specific to breaking down hydrogen peroxide, and hydrogen peroxide is an acid, so catalase should work best in an acidic environment.

EXPERIMENT:

Independent variable: PH - PH 3, 5, 7, 9 and 11 buffer solutions

Constants: Amount of catalase (potato), hydrogen peroxide

Apparatus required: Conical flasks-5, rubber corks, delivery tube, tube.

Experiment: 1. Pour distilled water into a clean boiling tube until it is half full. Place the fine open end of the delivery tube into the water so that the opening is 1 cm below the upper surface of water.

2. Measure 1 cm3 of buffer solution of different pH into previously labelled clean conical flasks, using a graduated pipette.

3. Measure 10 cm3 of the hydrogen peroxide carefully into each conical flask.

4. Set the stopclock to zero.

5. Measure 1 cm3 of the catalase solution, pour rapidly into the conical flask labelled pH 3.

6. Immediately place the delivery tube bung into the flask, swirl the contents and start the stopclock.

7. Measure the rate of oxygen production by timing the release of 10 bubbles from the end of the delivery tube. Swirl the contents continuously whilst timing the bubbles. Record your results.

8. Repeat the experiment for each of the different pH conditions.

9. Tabulate the rate of oxygen production per minute in each different pH solutions.

The amount of oxygen production increases fro pH 3 to 7 and then decreases by 11.

CONCLUSION: The table shows the rate of oxygen production at different pH solutions. The pH in which maximum oxygen production has occured is the pH at which catalase activity was more and has breaken more hydrogen peroxide.

Acidity affects proteins by changing the state of dissociable amino acid side chains. This change affect the catalytic property of enzyme.

References:

1. Google.com

2. www.madsci.org

3. Jes Stj. Effect of pH on catalase activity. 25 March 2013. 1466.

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