VEGF-A null mice die during embryogenesis and never get far enough along to deve

Question

VEGF-A null mice die during embryogenesis and never get far enough along to develop the circulatory system. Researchers developed a line of mice expressing Cre recombinase, that could selectively shut off VEGF-A in limb skin. They found that the initial capillary network aligned with the nerves much the same way as it did in normal mice, but vessels failed to differentiate into arteries. So does this support the hypothesis that VEGF-A is the molecule that organizes the arterial network and causes its differentiation?

Explanation / Answer

Cre recombinase acts tissue specifically and time specific,Cre- Lox system is v.much specific,the enzyme after getting activated shut off VEGFA specifically in limb,

platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family =encodes a protein that is often found as a disulfide linked homodimer. acts on endothelial cells and has various effects, mediating increased vascular permeability, Inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.
effects on a number of other cell types stimulation monocyte/macrophage migration, neurons, kidney epithelial cells. In vitro, VEGF-A stimulate endothelial cell mitogenesis and cell migration.vasodilator and increases vascular permeability factor.DNA encoding the gene of interest is integrated into a vector along with elements that are able to promote the gene’s expression. The vector is then injected either into muscle cells of the heart or the blood vessels supplying the heart into the limb, then it can be seen that differentiation taking place in arteries

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