suppose you would like to study a gene that codes for a peptide hormone H secret

Question

suppose you would like to study a gene that codes for a peptide hormone H secreted by human neurons. you know the primary sequence of this peptide hormone. explain how you might be able to identify or isolate the gene that codes for this peptide hormone. how will you produce multiple copies of this gene for study? how will you produce large quantities of this hormone for evaluation as a potential therapeutic age? provide a detailed outline of steps you will use for each of these objectives explaining why that step is needed.

Explanation / Answer

We can identify the gene by designing a complimentary probe for the peptide since we know its sequence, this probe will bind to the peptide and tell us. this probe could be a radioactive one so that we can isolate it better. for isolating it, we can use restriction digestion enzymes, that can cut at specific sites and give us fragments of DNA. We can produce multiple copies of this gene by using PCR, which amplifies a specific DNA sequence. we can then put our separated DNA sequence inside a vector inside a bacteria so that it copies as the bacteria divides and produce enough to be used for therapeutic purposes.

Related Questions

Navigate

• Browse (All)
• Browse S
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.