Please help with 10, 11, and 12 10) Match each protein or subunit to its major r

Question

Please help with 10, 11, and 12 10) Match each protein or subunit to its major role in DNA replication. (1 pt each) keeps template in ssDNA form. A. PCNA B. Ligase C. -sliding clamp D. SSB E. DNA polymerase F. Primase G. DNA polymerase I H. Gyrase cuts the DNA template to control supercoiling digests the primer of okazaki fragments makes RNA primers for lagging strand synthesis joins okazaki fragments together eukaryotic DNA sliding clamp binds the polymerase to the DNA template replicates long nucleic acid template 11) Choose the best match for each of the following terms with their role in transcription. (1 pt each) A. Enhancer B. Sigma factor C. Promoter D. Regulatory element E. RNA Poll F. RNA Pol G. Transcription factor H. RNA Pol protein that binds to a regulatory element promoter module for environment-sensing genes transcribes rRNA genes a site that RNA Pol binds to initiate transcription , a distant stimulatory gene sequence transcribes mRNA allows for recognition of different DNA promoters transcribes tRNA

Explanation / Answer

10
A. PCNA-eukaryotic DNA sliding clamp
B. Ligase-joins okazaki fragments together
C. beta-sliding clamp-binds the polymerase to the DNA template
D. SSB-keeps template in ssDNA form
E. DNA polymerase delta-digests the primer of okazaki fragments
F. primase-makes RNA primers for lagging strand synthesis
G. DNA polymerase I-replicates long nucleic acid template
H. Gyrase-cuts the DNA templateto control supercoiling

11
A. enhancer-promoter module for environment sensing genes
B. sigma factor-a site that RNA Pol binds to intiate transcription
C. promoter-allows for recognition of different DNA promoters
D. regulatory element-a distant stimulatory gene sequence
E. RNA Pol II-transcribes mRNA
F. RNA Pol III-transcribes tRNA
G. Transcription factror-protein that binds to a regulatory element
H. RNA Pol I-transcribes rRNA genes

12.
A.30S subunit-prokaryotic protein that helps binds AA-tRNA at A site for loading onto correct mRNA codon
B.50S subunit-site of the decoding center
C.EF-Ts-protein that replaces GDP with GTP on EF-Tu
D.EF-Tu-prokaryotic protein that helps binds AA-tRNA at A site

E. IF-3-protein that binds at the E site to prevent 50S binding

F. RF-1-protein that binds A site nonsence codon for termination

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